Table 1 RT-qPCR primer used for amplification.
Target | Sequences | Product length (bp) |
|---|---|---|
P53 | Forward: 5ʹ-TCACAGTCGGATATCAGCCT-3ʹ | 172 |
Reverse: 5ʹ-ACACTCGGAGGGCTTCACTT-3ʹ | ||
H2AX | Forward: 5ʹ-AGCGACTCAACTACAACCCAAACA-3ʹ | 70 |
Reverse: 5ʹ-AGGCTCAGTCCAGACAGGGATT-3ʹ | ||
P21 | Forward: 5ʹ-ACTTCCTCTGCCCTGCTGC-3ʹ | 141 |
Reverse: 5ʹ-GCTGGTCTGCCTCCGTTTT-3ʹ | ||
p16Ink4a | Forward: 5′-CAGGTGATGATGATGGGCAACG-3′ | 146 |
Reverse: 5′-TGCAGCACCACCAGCGTGTC-3′ | ||
Sirt1 | Forward: 5ʹ-GCCACCAACACCTCTTCATA-3ʹ | 233 |
Reverse: 5ʹ-TACTGGAACCAACAGCCTTA-3ʹ | ||
Lamin B1 | Forward: 5ʹ-GGGAAGTTTATTCGCTTGAAGA-3ʹ | 62 |
Reverse: 5ʹ-ATCTCCCAGCCTCCCATT-3ʹ | ||
c-Fos | Forward: 5ʹ-GGGACAGCCTTTCCTACTACC-3ʹ | 88 |
Reverse: 5ʹ-AGATCTGCGCAAAAGTCCTG-3ʹ | ||
Nanog | Forward: 5ʹ-GCAGAAGTACCTCAGCCTCC-3ʹ | 110 |
Reverse: 5ʹ-ACCGCTTGCACTTCATCCTT-3ʹ | ||
Myc | Forward: 5ʹ-TGACCTAACTCGAGGAGGAGCTGGAATC-3ʹ | 170 |
Reverse: 5ʹ-AAGTTTGAGGCAGTTAAAATTATGGCTGAAGC-3ʹ | ||
Oct4 | Forward: 5ʹ -TGTTCAGCCAGACCACCATC-3ʹ | 100 |
Reverse: 5ʹ-GCTTCCTCCACCCACTTCTC-3ʹ | ||
Sox2 | Forward: 5ʹ-AGGAGAGAAGTTTGGAGCCC-3ʹ | 152 |
Reverse: 5ʹ-TCTGGCGGAGAATAGTTGGG-3ʹ | ||
Klf4 | Forward: 5ʹ-GTGCCCCGACTAACCGTTG-3ʹ | 185 |
Reverse: 5ʹ-GTCGTTGAACTCCTCGGTCT-3ʹ | ||
IL1α | Forward: 5′-AGGAGAGCCGGGTGACAGTA -3′ | 51 |
Reverse: 5′-TCAGAATCTTCCCGTTGCTTG -3′ | ||
IL1β | Forward: 5′-CCAAAAGATGAAGGGCTGCT-3′ | 51 |
Reverse: 5′-TCATCAGGACAGCCCAGGTC-3′ | ||
IL6 | Forward: 5′-CTGCAAGAGACTTCCATCCAG-3′ | 131 |
Reverse: 5′-AGTGGTAGACAGGTCTGTTGG -3′ | ||
IL8 | Forward: 5′-CTGGTCCATGCTCCTGCTG-3′ | 51 |
Reverse: 5′-GGACGGACGAAGATGCCTAG-3′ | ||
MMP3 | Forward: 5′-ACTCCCTGGGACTCTACCAC-3′ | 163 |
Reverse: 5′-GGTACCACGAGGACATCAGG-3′ | ||
MMP13 | Forward: 5′-GATGGACCTTCTGGTCTTCT -3′ | 136 |
Reverse: 5′-GCTCATGGGCAGCAACAATA -3′ | ||
β⁃actin | Forward: 5ʹ-CTGTGCCCATCTACGAGGGCTAT-3ʹ | 155 |
Reverse: 5ʹ-TTTGATGTCACGCACGATTTCC-3ʹ |
