Fig. 3
Functional impact of m 6 A-associated SNPs in colon cancer. (A) Genomic distribution of colon cancer-associated SNPs overlapping with increased m6A peaks. We define these SNPs as m6A-associated SNPs in colon cancer. (B) Chromosomal distribution of m6A-associated SNPs. (C) Annotation of m6A-associated SNPs using eQTLs and sQTL information based on snpXplorer48. Red box indicates the presence of eQTLs and/or sQTLs of corresponding SNPs. (D) Expression of RIBC2 and MCM3AP-AS1 in tumor vs. normal tissues based on RNA-seq data. Statistical significance was determined based on adjusted P-values (Wald test in DESeq2 followed by Benjamini-Hochberg multiple correction). (E) Evaluation of alternative splicing events for five sQTL-related genes detected using rMATS-turbo. Red indicates the presence of alternative splicing events of corresponding genes in colon cancers compared to normal. The asterisks indicate statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.
