Fig. 4
Orthogonal validation of differentially expressed genes and differential splicing events in colon cancer potentially linked with m6A-associated SNPs. (A) RT-qPCR showing increased expression of RIBC2 and MCM3AP-AS1 after ALKBH5 knockdown in DLD1 and CCD841 cells. Statistical significance was determined using two-tailed unpaired t-test. Error bars represent standard deviation (SD). (B) Upregulation of RIBC2 and MCM3AP-AS1 in colon tumor tissues compared to normal tissues based on orthogonal RNA-seq dataset (PRJNA742008). Statistical significance was determined based on adjusted P-values (Wald test in DESeq2 followed by Benjamini-Hochberg correction). (C) Global identification of differential splicing events between tumor and normal tissues categorized by event type. (D) Alternative splicing events of selected genes identified from the PRJNA742008 dataset using rMATS-turbo. Red boxes indicate the presence of alternative splicing events of corresponding genes in colon cancers compared to normal. The asterisks indicate statistical significance: * P < 0.05, ** P < 0.01.
