Table 2 Group, sequence and cycles characteristics for bacterial (16 S), fungal internal transcribed spacer (ITS) and oomycetes (ITS) amplification.
Group | Sequence | Cycle | References | |
|---|---|---|---|---|
16 S (V4) | 515 F 806R | GTGYCAGCMGCCGCGGTAA GACTACHVGGGTWTCTAAT | 30 cycles: 95 °C 30s, 55 °C 30s, 72 °C 30s | |
ITS | ITS1f ITS2 | CTTGGTCATTTAGAGGAAGTAA GCTGCGTTCTTCATCGATGC | 30 cycles: 95 °C 30s, 55 °C 30s, 72 °C 30s | |
ITS-oomycetes | ITS6 ITS7ae | GAAGGTGAAGTCGTAACAAGG WGYGKTCTTCATCGATGTGC | 30 cycles: 95 °C 30s, 55 °C 30s, 72 °C 30s |
