Fig. 2

Transcriptomics with GO and KEGG^54,55 pathway enrichment analyses. Top 20 enriched biological process-related terms in GO enrichment analysis with DEGs upregulated greater than twofold (a) in CHL and CHL-IgG cells against parental CHO and CHO-K1-IgG cells, (b) in parental CHO and CHO-K1-IgG cells against CHL and CHL-IgG cells, (c) in CHL-IgG cells against CHO-K1-IgG cells, and (d) in CHO-K1-IgG cells against CHL-IgG cells. Significantly enriched terms in KEGG pathway enrichment analysis with DEGs upregulated greater than twofold (e) in CHL and CHL-IgG cells against parental CHO and CHO-K1-IgG cells, (f) in parental CHO and CHO-K1-IgG cells against CHL and CHL-IgG cells, (g) in CHL-IgG cells against CHO-K1-IgG cells, and (h) in CHO-K1-IgG cells against CHL-IgG cells. In the figures, the size of plots, the color of plots, and the x-axis indicate the number of DEGs categorized in each term, FDR transformed using -log₁₀, and p-value transformed using -log₁₀, respectively. P-values represent the raw statistical significance of enrichment, while q-values indicate the FDR-adjusted significance. Terms with p < 0.01 and q < 0.1 were considered significantly enriched in exploratory analysis. The use of the KEGG database in this study was conducted under authorization granted by Kanehisa Laboratories^54,55.