Fig. 5
A p190A regulates LIMK/Cofilin and FAK/cortactin pathways in BC cells. (A) Western blots show that the expression of RhoA, ROCK1, phospho-LIMK1/2 (p-LIMK1/2), and phospho-cofilin1 (p-cofilin1) are upregulated upon p190A knockdown in RT4 cells using three different shRNAs (shp190A-1, shp190A-2, shp190A-3), while total cofilin and LIMK levels remained unchanged. Similarly, phospho-cortactin (p-cortactin) and phospho-FAK (p-FAK) levels are increased in cells with p190A knockdown but the total cortactin and FAK levels remain unchanged. (B) Western blots show that the expression of RhoA, ROCK1, phospho-LIMK1/2 (p-LIMK1/2), and phospho-cofilin1 (p-cofilin1) are downregulated upon ectopic p190A expression in BFTC cells (p190A-OE), while p190A overexpression did not influence total cofilin and LIMK levels. Similarly, phospho-cortactin (p-cortactin) and phospho-FAK (p-FAK) levels are downregulated upon p190A overexpression (BFTC-OE) but the total cortactin and FAK levels remain unchanged. α-tubulin, β-actin and GAPDH were used as loading controls. Please note that for all Western blots (WB), full-length gels are provided in the supplemental data file (Suppl. Figure 2). Quantification of all WBs shown in Fig. 5, including full gels of the WBs are provided as supplemental data (Suppl. Figure 3). The Western blots in Figs. 1, 3 and 5 were repeated at least for three times (Suppl. Figure 7). Please note that due to the experimental requirements, i.e. multiple use of the membranes with different antibodies, different loading controls were used in some cases in Figs. 1, 3 and 5.
