Table 1 Tumor features and CTC detection of HCC patients.

From: A fixation-compatible protocol for intracellular and surface marker-based detection of circulating tumor cells in hepatocellular carcinoma

Patient ID

Blood Collection Time

BCLC stage

Number of lesions

Largest lesion size (cm)

CTC

(per 5 ml)

CTNNB1 Codon 32–37 Mutation%

Plasma cfDNA

CTC DNA

HCCAK503

Pre-treatment

C

10

5.6

16

NA

NA

HCCAK516

Pre-transplantation

A

1

3.3

11

NA

NA

HCCAK519

Pre-transplantation

B

3

3.4

2

NA

NA

HCCAK484

Post-TACE

B

1

2.8

2

NA

NA

HCCAK521*

Pre-TACE

B

4

4

6

BLOD (QNS)+

1.14%, 1.25%

HCCAK526*

Pre-TACE

B

1

6.4

28

BLOD (QNS)+

0.48%, 1.12%

HCCAK505

Post-TACE

0

1

1.6

11

NA

NA

HCCAK520

Pre-TACE

A

1

3

6

NA

NA

HCCAK493

Pre-TACE

B

1

5.3

3

NA

NA

HCCAK489

Pre-transplantation

B

1

2.4

7

NA

NA

  1. *CTC and plasma DNA samples from these two patients were tested using ddPCR with a gene mutation panel.
  2. +QNS, quantity not sufficient due to low DNA concentration, < 500 copies DNA input was used for assay. For CTNNB1 Codon T41A and S45F, both mutations were undetected due to QNS in cfDNA and CTC DNA samples.
  3. The mutation% shown were calculated by the total mutant copies over the total CTNNB1 copies. These two results were verified in a repeat run.