Fig. 7 | Scientific Reports

Fig. 7

From: Comprehensive analysis of single-cell and bulk RNA sequencing uncover tumor microenvironment diversity in invasive Retinoblastoma

Fig. 7The alternative text for this image may have been generated using AI.

The detection of module genes using weighted gene co-expression network analysis (WGCNA) in invasive and non-invasive RB. A Outlier samples were removed from the analysis of invasive versus non-invasive groups. B Clustering was performed using gene expression data, with color intensity representing disease status (invasive vs. non-invasive). C In the gene tree, different colors denote distinct gene co-expression modules. E A collinear heatmap of module feature genes is shown, where red indicates high correlation and blue represents low or inverse correlation. D DOK7 expression in Y79 cell e was quantitatively analyzed by qPCR. E Proliferation attenuation mediated by DOK7 knockdown was validated through CCK-8 viability assay (72-h time course). F Colony formation capacity assessment confirmed the anti-proliferative effect of DOK7 silencing . G Wound healing assay demonstrated impaired migratory potential in DOK7-deficient cells (0/48-h monitoring). H The Transwell migration chamber (upper) and Matrigel invasion assays (lower) jointly verified the suppressive effect of DOK7 silencing on both migratory and invasive capacities (24-h incubation).

Back to article page