Fig. 1

HDAC6 expression in ALL cell lines and the effect of WT161 on HDAC6, acetyl-α-tubulin, and cell proliferation. (a) HDAC6 protein expression in four ALL cell lines (BALL-1, NALM-6, Jurkat, and MOLT-4) as detected by Western blot. (b) Relative HDAC6 mRNA levels measured by quantitative PCR (qPCR), showing expression trends consistent with (a). (c) Western blot analysis of HDAC6 and acetyl-α-tubulin protein levels in cell lines treated with or without 5 µM WT161 for 72 h. (d) Concentration- and time-dependent inhibition of cell proliferation by WT161 across all four ALL cell lines. IC50 values were calculated to quantify compound sensitivity.