Table 7 Effect of supplementing LEO (free and nanoencapsulated) on cecal microbial population at 42 days post hatch in broiler chicken.

Parameter¹ (Log₁₀ CFU/g)	Dietary treatments²								SEM	P-value
Parameter¹ (Log₁₀ CFU/g)	CN	AB	CS	LEO_F200		LEO_F400	LEO_N200	LEO_N400	SEM	P-value
TPC	8.87^a	8.54^c	8.79^ab		8.71^abc	8.73^abc	8.62^bc	8.59^bc	0.03	0.019
Coliform count	6.89^a	6.48^bc	6.73^ab		6.55^bc	6.51^bc	6.32^c	6.27^c	0.05	0.004
LAB count	5.63^bc	5.45^c	5.57^bc		5.77^ab	5.79^ab	5.89^a	5.92^a	0.04	0.005

Means in a row with different superscripts differ significantly (P < 0.05).
1CFU, Colony forming unit; TPC, Total plate count; LAB, Lactobacillus.
²CN (Control)-fed basal diet only; AB (Antibiotic)-basal diet + 10 mg/kg Enramycin; CS (Chitosan)-basal diet + 300 mg/kg chitosan nanoparticles; LEO_F200 (basal diet + 200 mg/kg free LEO); LEO_F400 (basal diet + 400 mg/kg free LEO); LEO_N200 (basal diet + 200 mg/kg nanoencapsulated LEO) and LEO_N400 (basal diet + 400 mg/kg nanoencapsulated LEO).

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