Fig. 1
Candida albicans effect in melanoma cells phenotype. Melanoma cells migration after 24 h of exposure for six hours to (a) live-C. albicans yeast MOI 0.25 and 0.5 or (b) Heat-Killed (HK)-C. albicans yeast MOI 10. (c) LSECs migration after eight hours of exposure to the conditioned media (CM) collected from melanoma cells exposed to live C. albicans MOI 5 of the fungus for six hours. (a–c) graphs represent all the results obtained and images (10X magnification) are representative of one experiment of each assay (n = 9 technical replicates from 3 biologically independent samples). Scale bars 100 μm. (d,e) Effect of C. albicans stimulation of melanoma cells on liver metastasis promotion (n = 13 biologically independent samples): (d) Representative macroscopic liver images, and graphical representation of liver area occupied by metastatic melanoma in control group mice and C. albicans-stimulated melanoma cells receiving mice. (e) Representative H&E staining of metastatic colonization, and microscopic tumor area size (in mm2) per 200 mm2 of liver section in control group and C. albicans-stimulated melanoma cells injected group. Scale bar 500 μm. Data were presented as the individual values, means ± SEM. Two-tailed unpaired Student’s t test was used to examine the statistical significance between groups. *, **, and **** denote P < 0.05, P < 0.01, and P < 0.001, respectively.
