Fig. 5 | Scientific Reports

Fig. 5

From: Peptidylarginine deiminase 2 contributes to pathogenesis in trinitrobenzenesulfonic acid-induced colitis through macrophage extracellular trap-independent pathways

Fig. 5

Macrophage polarization and inflammatory mediator expression in intraperitoneal macrophages derived from PAD2KO mice. (a,b) Macrophage polarization was determined using flow cytometry. CD11b-positive and F4/80-positive cells were sorted from the live cells in the population of intraperitoneal macrophages, and M1 and M2 macrophages were then determined by CD80-positive cells (M1) or CD163-positive cells (M2) from the sorted population. (c) CD86, (d) iNOS, (e) CD206, (f) arginiase-1 (Arg-1), (g) TNFα, (h) IL-1β, (i) IL-12, (j) IL-23, and (k) CXCL2 mRNA expression were determined using qRT-PCR in unstimulated intraperitoneal macrophages from WT and PAD2KO mice, and mRNA levels were normalized to TBP. Data are presented as the means ± S.E.M. (n = 7–8). ****P < 0.0001, **P < 0.01 vs. WT macrophages.

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