Fig. 2
Intraperitoneal transplantation of Tokyo bitterling testicular cells into oily bitterling larvae. (A) Fluorescence image of Tokyo bitterling testicular cells isolated and fluorescently labelled with PKH26. BF shows a bright field view. Bars: 20 µm. (B) Intraperitoneal transplantation of testicular cells into oily bitterling larvae. (C) Fluorescence images of the immature gonads of oily bitterling recipients at 18 days after transplantation. Transplanted, germ cell-less control, and wild-type represent the gonads of dnd-KD recipients receiving donor germ cells, those of dnd-KD recipients without transplantation, and those of wild-type controls, respectively. The white arrowheads indicate donor-derived germ cells labelled with PKH26. The yellow arrowheads indicate endogenous germ cells with round-shaped nuclei. Bars: 10 µm.
