Fig. 1 | Scientific Reports

Fig. 1

From: High-resolution native electrophoresis in-gel activity assay reveals biological insights of medium-chain fatty acyl-CoA dehydrogenase deficiency

Fig. 1

Octanoyl-CoA:NBT oxidoreductase in-gel activity of human recombinant MCAD WT. (A) High-resolution clear native (hrCN) gels after electrophoretic separation of human recombinant MCAD WT at pH 7.0. From left to right: Coomassie blue stained gel; Intrinsic FAD fluorescence; and C8-CoA:NBT oxidoreductase in-gel activity. (B) Quantification profiles of the main oligomer band. (C) Integrals from the quantification peaks were plotted as a function of the protein amount per lane. This figure shows a representative gel (n = 3).

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