Fig. 2 | Scientific Reports

Fig. 2

From: Extracellular mitochondrial DNA activates complement and is associated with complement activation in patients with out-of-hospital cardiac arrest

Fig. 2

A mtDNA induced complement activation in whole blood and plasma. Human whole blood (A-C) or plasma (D-F) was incubated with increasing concentrations of a mtDNA-enriched fraction and nDNA-fraction. C3bc (A, D), C3bBbP (B, E), and sC5b-9 (C, F) increased in a dose- and time-dependent manner of the mtDNA-enriched fraction and zymosan (100 µg/ml) serving as positive control. Thus, significant increases in the presence of mtDNA and zymosan were observed for C3bc (A, D) and sC5b-9 (C), whereas nDNA had no effect on complement activation. Data are presented as mean ± SEM. sC5b-9 data points from one donor were excluded (due to technical issues); A,D (n = 6), B, E (n = 6), and C, F (n = 5). General mixed model analyses. * = p < 0.05, *** = p < 0.001. CAU; complement arbitrary units.

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