Fig. 2
Schematic of CAR T cell expansion protocol and sampling strategy. Cultures were generated from three healthy donors. T cells were isolated from donor buffy coat samples and activated using CD3/CD28 Dynabeads™ and IL-2 at 1 × 106 cells/mL. On day 1 the T cells were infected with Lentivirus containing a CD19 CAR. On day 3, supernatants were sampled for 1H NMR and the cell diluted to 0.5 × 106 cells/mL. This procedure was repeated every second day. Beads were removed on day 6, and IL-2 supplementation followed medium change. Cells were collected on day 1, 7, 14, and 21 for flow cytometry and dDNP-NMR analysis.
