Fig. 2

Myelin phagocytosis is altered in macrophages lacking CD200R1. (A,B) Gating strategy (continued from Fig. 1A) and flow cytometry assay scheme of pHrodo-myelin phagocytosis by cell populations obtained from the spinal cord tissue after injury. (C) Percentage of pHrodo⁺ microglia at 1, 3 and 7 days post-SCI. (D) Percentage of pHrodo⁺ macrophages at 1, 3 and 7 days post-SCI. (Two-way ANOVA followed by Bonferroni post-hoc test, *p ≤ 0.05). (E, F) Gating strategy and In vitro assay scheme of pHrodo-labeled myelin phagocytosis by BMDMs from WT and CD200R1^−/− mice. (G) Quantification of the proportion of pHrodo⁺ WT and CD200R1^−/− BMDM in basal conditions and following 24 h of exposure to LPS (H) Quantification of pHrodo median fluorescence intensity in WT and CD200R1^−/− BMDM in basal conditions and following 24 h of exposure to LPS. Each symbol represents one animal. Data are presented as mean ± SEM. For the in vitro experiments, each condition was performed in triplicate from cultures prepared from two independent animals per group (Two-way ANOVA followed by Bonferroni post-hoc test, *p ≤ 0.05, ***p ≤ 0.001).