Fig. 3
Autogamy in D. incarnata. (A) 24 HAP, megaspore mother cell at prophase I. (B) 24 HAP, division in the chalazal cell of the dyad (chd); the micropylar cell of the dyad (md) degenerates. (C) 48 HAP, end of megasporogenesis; triad of megaspores; this enlarged and chalazaly positioned becomes the functional megaspore (FM). (D) 48 HAP, early megagametogenesis: two nucleate FG; the remnants of degenerated megaspores are marked by a star. (E) 48 HAP, four-nucelate FG; three of four nuclei are marked by arrowheads. (F) 10 DAP; mature female gametophyte with the egg cell (ec) and the polar nucleus (pn). (G) 10 DAP, a few-cell embryo (em). (H) 21 DAP; globular embryos (arrows) inside ovary. mmc-megaspore mother cell; ii-inner integument; oi-outer integument; chd-chalazal cell of the dyad; md-micropylar cell of the dyad; ch-chalazal megaspore of the triad; FM-functional megaspore; sch-subchalazal megaspore of the triad; m-micropylar megaspore of the triad; FG-female gametophyte; ec-egg cell; pn-polar nucleus; em-embryo. Bar = 20 µm for (A–G) images.
