Fig. 4
From: Quantitative holographic analysis in stallion spermatozoa following cryopreservation

Sperm volume assessment. Sperm volume assessment conducted for three analyzed regions corresponding to different refractive index ranges: (a) Post-acrosomal region and Midpiece, (b) Nuclear region, and (c) Whole cell. The box plots on the left illustrate individual variations in fresh stallion sperm, while those on the right depict extender-related variations in fresh and frozen/thawed sperm, independent of individual variation.