Fig. 1

Characterization of EPIC-derived EVs. (A) Representative TEM images of EVs-N (i), EVs-H5% (ii), and EVs-H1% (iii) samples. Scale bar: 50 nm; (B) Particle concentration (number of particles/mL) of EVs-N (7.7 ± 2.3 × 10¹¹ particles/mL), EVs-H5% (3.5 ± 0.5 × 10¹¹ particles/mL) and EVs-H1% (2.8 ± 0.4 × 10¹¹ particles/mL); (C) Modal sizes of EVs-N (142.2 ± 25.5 nm), EVs-H5% (136.5 ± 13.1 nm) and EVs-H1% (103.8 ± 10.5 nm); (D) Distribution of particle population in three different size ranges from EVs-N (35–200 nm: 73.8 ± 2.2%; 200–400 nm: 19.6 ± 1.9%; > 400 nm: 2.5 ± 0.6%), EVs-H5% (35–200 nm: 80.1 ± 3.3%; 200–400 nm: 17.8 ± 1.9%; > 400 nm: 2.1 ± 0.4%) and EVs-H1% (35–200 nm: 86.4 ± 1.7%; 200–400 nm: 12.3 ± 1.6%; > 400 nm: 1.3 ± 0.3%); (E) Representative images of western blot against ALIX (95 kDa) and TSG101 (45 kDa) accumulation in protein lysates from total mouse embryos at E11.5 (control), EVs-N, EVs-H5%, EVs-H1%. Data are presented as mean ± SEM, n = 3. One-way ANOVA test for particle concentration and modal size. Two-way ANOVA test for particle size distribution, *p < 0.05.