Fig. 1 | Scientific Reports

Fig. 1

From: Ethylenediaminetetraacetic acid conjugated with polyethylene glycol is a safe chelator for colonic epithelial denudation and intestinal epithelial replacement therapy

Fig. 1

Synthesis and characterization of E-PEGs. (a) Monomethoxy PEG (mPEG) has a methoxy group at one end. EDTA monoanhydride and EDTA dianhydride were reacted with mPEG to synthesize one- and two-PEG-conjugated E-PEGs, respectively. Reaction of EDTA monoanhydride with mPEG of mean MWs of approximately 550, 2,000, or 4,000 Da yielded E-PEG1, E-PEG2, or E-PEG3, respectively. Reaction of EDTA dianhydride with mPEG of mean MW of approximately 2,000 Da yielded E-PEG4. (b) UHPLC-QTof-MSE analysis of the mixed solution of E-PEG1 and Ca. The scheme on the right shows the chemical formulas and transitions of protons during complex formation and MS analysis. The theoretical molecular weights of E-PEG1 with different number of oxyethylene repeats (n) are as follows: 827.3338 for C33H59N2O19Ca (n = 11), and 783.3076 for C31H55N2O18Ca (n = 10). The discrepancy between the theoretical and measured values ​​was negligible.

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