Fig. 4 | Scientific Reports

Fig. 4

From: CD38-specific nanobody-based bispecific antibody recruiters (BARs) redirect complement-dependent cytotoxicity toward multiple myeloma cells

Fig. 4

Nanobody-based CD38-specific BARs induce antibody-dependent cellular cytotoxicity (ADCC) against CD38-expressing cell lines in vitro. Viability of Daudi luc (left), CA-46 luc (middle), and LP-1 luc cells (right) after incubation with 100 nM of CD38-BARs. NK92 cells were added at an effector-to-target ratio of 3:1 and incubated with tumor cells for 180 min. 25% inactivated human pooled serum was used as a source of antibodies. Daratumumab served as a positive control. Ctrl-BAR and cells incubated without BAR served as negative controls. Depicted are means ± SD of three parallel replicates. Results of a two-way ANOVA between ctrl-BAR and BARs are indicated as p-values above the diagrams.

Back to article page