Fig. 5

Assessment of platelet basal activation state and its functional competence in ITP patients with respect to bleeding score. (A) Analysis of platelet counts conducted between BS1 (blue) and BS2 (red). Furthermore, (B) the MPV, PLCR, RP, and PDW indices were evaluated between these groups. The BS groups were also analyzed according to (C) P-selectin expression, (D) PAC-1 binding, and (E) ROS generation at baseline and in response to an agonist in comparison with healthy controls (gray). Black asterisks illustrate statistical differences between BS1 and 2 groups. Red asterisks show statistical differences of ITP (n = 28) vs., the related control (n = 20). *P <.05, **P <.01, and ***P <.001. Data presented as mean ± SD. CCCP, carbonyl cyanide m-chlorophenylhydrazone; MPV, mean platelet volume; PDW, platelet distribution width; P-LCR, platelet larger cell ratio; PMA, Phorbol 12-Myristate 13-Acetate; ROS, Reactive Oxygen Species.