Table 1 Summary of read processing and de novo assembly statistics for cassava leaf samples sequenced using Oxford Nanopore technology.

From: Improving the diagnosis of cassava mosaic begomoviruses using Oxford Nanopore Technology sequencing

Accession

Bioproject accession

Biosample accession

Reads raw

reads_host mapped

reads_host unmapped

Read N50_bp

Read max_bp

ctg_count

Total bp_contigs

ctg_total_bp

ctg_N50_bp

SRR35173598

PRJNA1312059

SAMN50848258

24,876

23,282

1,594

5,405

5,561

4

17,929

5,561

5,405

SRR35173597

PRJNA1312059

SAMN50848259

19,604

7,855

11,749

3,352

5,650

10

21,899

5,650

3,352

SRR35173596

PRJNA1312059

SAMN50848260

32,167

30,188

1,979

3,012

4,680

6

16,065

4,680

3,012

SRR35173595

PRJNA1312059

SAMN50848261

36,085

33,674

2,411

3,585

7,510

14

41,710

7,510

3,585

SRR35173594

PRJNA1312059

SAMN50848262

21,456

20,128

1,328

3,610

3,713

3

10,134

3,713

3,610

SRR35173593

PRJNA1312059

SAMN50848263

11,660

9,800

1,860

5,961

8,172

6

27,386

8,172

5,961

SRR35173592

PRJNA1312059

SAMN50848264

28,450

26,706

1,744

8,223

8,343

6

29,343

8,343

8,223

SRR35173591

PRJNA1312059

SAMN50848265

10,442

4,894

5,548

22,248

22,248

2

41,323

22,248

22,248

SRR35173590

PRJNA1312059

SAMN50848266

7,838

3,673

4,165

13,905

13,905

5

20,292

13,905

13,905

SRR35173589

PRJNA1312059

SAMN50848267

12,533

10,160

2,373

10,904

10,904

2

16,466

10,904

10,904

  1. The table reports the number of raw reads generated per barcode, the number of reads mapped to the cassava or human reference genomes, and the number of non-host/non-human reads retained for viral analysis. Read-level statistics include the number of final reads, N50 length and maximum read length. Assembly-level statistics include the number of contigs produced, total contig length, maximum contig length and contig N50. These metrics provide an overview of sequencing depth, host read removal efficiency and assembly quality for downstream viral genome reconstruction and taxonomic assignment. (http://www.ncbi.nlm.nih.gov/bioproject/1312059).