Table 4 Previously published primer pairs for the detection of cassava mosaic begomoviruses (CMBs).

From: Improving the diagnosis of cassava mosaic begomoviruses using Oxford Nanopore Technology sequencing

Primer name

Primer sequence

Size (bp)

Target region

References

JSP001F

ATGTCGAAGCGACCAGGAGAT

783

AV2 (CP)

Pita et al.4

JSP002R

TGTTTATTAATTGCCAATACT

ACMVBF

TCGGGAGTGATACATGCGAAGGC

628

AV1/BC1

Matic et al.16

ACMVBR

GGCTACACCAGCTACCTGAAGCT

JSP001F

ATGTCGAAGCGACCAGGAGAT

780

AV2 (CP)

Pita et al.4

JSP003R

CCTTTATTAATTTGTCACTGC

CMBRepF

CRTCAATGACGTTGTACC A

650

AC1

Alabi et al.17

EACMVRepR

GGTTTG CAGAGAACTACATC

VNF031F

GGATACAGATAGGGTTCCCAC

 ~ 560

AC2/AC3

Fondong et al.18

VNF032R

GACGAGGACAAGAATTCCAAT

EAB555F

TACATCGGCCTTTGAGTCGCATGG

544–560

BC1/CR

Ndunguru et al19.

EAB555R

CTTATTAACGCCTATATAAACACC

  1. These primers have been widely used in earlier studies for virus identification and epidemiological studies, providing a foundation for comparative analysis with newly designed primers in this study.
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