Fig. 4
Metabolomic profile of skeletal muscle in adenine and PN CKD models. (A) Terminal body weight in adenine CKD animals. Analysed by Student’s t-test, *p < 0.05, control n = 8, Adenine n = 10 (B,C) Plasma creatine kinase level in (B) adenine diet CKD animals (Analysed by Mann-Whitney U test, *p < 0.05, control n = 8, Adenine n = 10) and (C) PN CKD animals (Analysed by unequal variance t-test, p > 0.05, sham n = 7, PN CKD n = 7). (D, E) Muscle metabolomic profile measured by 1H NMR spectroscopy of (D) adenine diet CKD (n = 5) vs. control diet (n = 5) and (E) PN CKD (n = 3) vs. sham (n = 3). Statistical analysis displayed was performed using Student’s t-test comparison for each metabolite on untransformed metabolite levels (#p < 0.05); results and values are displayed in Tables S6 and S7. Data displayed as mean ± SEM. Metabolomic data displayed were acquired by 1H NMR spectroscopy using the aqueous phase containing water-soluble metabolites from a methanol/chloroform/water extraction protocol. Abbreviations: PN, partial nephrectomy; CKD, chronic kidney disease; NAD, nicotinamide adenine dinucleotide; ATP, adenosine triphosphate; ADP, adenosine diphosphate; PCr, phosphocreatine.
