Fig. 6
Metabolomic profile of liver in adenine and PN CKD models. (A, B) Liver weight in (A) adenine diet CKD (n = 10) vs. control diet (n = 8) animals and (B) PN CKD (n = 9) and sham (n = 6) animals. Analysed by Student’s t-test, *p < 0.05. (C, D) Liver metabolomic profile measured by 1H NMR spectroscopy of (C) adenine diet CKD (n = 5) vs. control diet (n = 4) and (D) PN CKD (n = 4) vs. sham (n = 4). Statistical analysis displayed was performed using Student’s t-test comparison for each metabolite on untransformed metabolite levels (#p < 0.05); results and values are displayed in Tables S10 and S11. Data displayed as mean ± SEM. Metabolomic data displayed were acquired by 1H NMR spectroscopy using the aqueous phase containing water-soluble metabolites from a methanol/chloroform/water extraction protocol. Abbreviations: PN, partial nephrectomy; CKD, chronic kidney disease; NAD, nicotinamide adenine dinucleotide; AMP, adenosine monophosphate.
