Fig. 2
From: Key features of the innate immune response is mediated by the immunoproteasome in microglia
Phagocytosis is impaired by immunoproteasome inhibition. WT and β5i KO BV-2 cells were treated for 24 h with ONX-0914 prior to measuring phagocytosis by flow cytometry. To adjust for background, control cells that were not exposed to fluorescent beads were used for each experiment. (A) Phagocytosis of IgG-coated latex beads was significantly different between groups using one-way ANOVA ([F(3,10) = 7.68, p = .005). Tukey’s HSD post hoc test revealed that treatment with ONX-0914 resulted in significantly decreased phagocytosis compared to control (p = .032). (B) Phagocytosis of eGFP-expressing E. coli was measured after a 30-minute incubation by quantifying the percentage of cells that were GFP positive. Flow cytometry analysis revealed a significant difference between treatment groups using one-way ANOVA ([F(3,18) = 39.23], p < .001). Tukey’s HSD post hoc test revealed that treatment with ONX-0914 resulted in significantly decreased phagocytosis compared to control (p < .001).
