Fig. 3
From: Demonstration of a novel method to explore osteon tilt in the human femoral cortex

A: ArcGIS Pro export of the composite of the superior femoral transverse section. Orienting grooves used for re-alignment of serial sections are circled in red. The orienting grooves were cut into the bone before sectioning and the three serial sections were overlaid and aligned using these grooves. B: ArcGIS Pro export of a close-up of the three layers polygons overlaid atop a composite micrograph of the superior transverse section. Blue osteons represent the superior layer, green osteons represent the middle layer, purple osteons represent the inferior layer. Composite micrographs of the middle and inferior sections are not shown for clarity. Mathematical centroids used to calculate osteon tilt are shown within the polygons.