Fig. 3

Photomicrograph of histological sections on the gills on Nile tilapia (Oreochromis niloticus). (a) Control group was conducted without any substance. (b, c) the gills of Oreochromis niloticus exposed to a low concentration (5%) of JA-NE showing partial fusion of secondary gill lamellae with infiltration by mononuclear inflammatory cells infiltration (black arrow), epithelial cell hyperplasia (red arrow). (d, e) the gills of Oreochromis niloticus exposed to a high concentration (10%) of JA-NE showing complete fusion of secondary gill lamellae (black arrow), congestion of blood vessels (red asterisk), epithelial lifting (red circle). Hematoxylin and Eosin stain, scale bar: a (100 µm), b–e (50 µm) (X400).