Fig. 4

Photomicrograph of histological sections on the livers of Nile tilapia (Oreochromis niloticus). (a) control group was conducted without any substance. (b, c) the livers of Oreochromis niloticus exposed to low concentration (5%) of JA-NE. (d, e) the livers of Oreochromis niloticus exposed to a high concentration (10%) of JA-NE. Cytoplasmic vacuolization (black arrow), congestion of hepatic blood sinusoids (red arrow), necrobiotic changes in hepatopancreas with congestion (red asterisk), pyknotic nucleus (green arrow). Hematoxylin and Eosin stain, scale bar: a: 100 µm, b–e: 50 µm (X400). All panels were captured at the same magnification and with the scale bars.