Fig. 1

In vitro scratch assay analysis. A) Representative images showing scratch closure after 20 hours (h) of co-culture of endothelial and smooth muscle cells at a 5:1 seeding ratio. Scale bars: 200 μm. B) Top: Percentage of scratch closure at 5 and 20 h, relative to baseline at 0 h. Bottom: Multiple comparison analysis using a mixed model to assess the effect of different plasma supplementation conditions at 0, 5, and 20 h. C) Percentage of scratch closure at 20 h. Statistical significance between plasma supplementation conditions is indicated.