Fig. 2 | Scientific Reports

Fig. 2

From: Hepatic vein-derived factors may affect pulmonary arteriovenous malformations after single ventricle palliation by modulating vascular cell behavior

Fig. 2

Arteriovenous tube formation assay. A) Representative images showing the effect of plasma supplementation from the superior vena cava (SVC), hepatic vein (HV), and a 1:1 mixture of SVC and HV (SVC + HV) compared to a negative control (no plasma) on arteriovenous endothelial tube formation after 6 hours (h) of culture. VE-CAD, VE-cadherin (green); DAPI, 4’,6-diamidino-2-phenylindole (blue). Scale bars: 500 μm. B) Quantification of tube formation after 6 h of culture. Tot. length, total network length; Nb junctions, number of junctions; Nb nodes, number of nodes. Superior vena cava (SVC), hepatic vein (HV), negative control (C–).

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