Fig. 2
Gnetin C reduces fat accumulation and promotes APN multimerization in adipose tissue. a-c Epididymal fat mass (a), subcutaneous fat mass (b), and total fat mass (c) levels in HFD-fed mice treated with vehicle, MSE (1,000 mg/kg/day), or Gnetin C (100 or 200 mg/kg/day) for 4 weeks. d-f Serum total APN (d), HMW APN (e), and LMW APN (f) levels, adjusted for fat mass, in HFD-fed mice treated with vehicle, MSE (1,000 mg/kg), or Gnetin C (100 or 200 mg/kg). g Relative mRNA expression levels of Dsba-l in adipose tissue of HFD-fed mice treated with vehicle, MSE (1,000 mg/kg), or Gnetin C (100 or 200 mg/kg). h, i Relative protein levels of PPARγ in adipose tissue of HFD-fed mice treated with vehicle, MSE (1,000 mg/kg), or Gnetin C (100 or 200 mg/kg). j Relative mRNA levels of genes involved in fatty acid synthesis (Acaca, Chrebp), adipose differentiation (Pparγ, Slc2a4), lipolysis (G0s2, Irf4), and fatty acid β-oxidation (Acox1, Pparα, Pgc1α) in adipose tissue from HFD-fed mice treated with vehicle, MSE (1,000 mg/kg), or Gnetin C (100, 200 mg/kg). Data are presented as mean ± SEM; 4–6 mice per group. Statistical significance was determined using ANOVA followed by Dunnett’s test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).
