Fig. 3
Effect of different doses of aprepitant on lung inflammatory parameters in hepatic ischemia reperfusion-induced lung injury in rats. Immunohistochemical expression of lung IL-1β in hepatic ischemia reperfusion induced lung injury in rats: Sham group (A), the Sham-Ap10 group (B), HIR group (C), Ap5 + HIR group (D), Ap10 + HIR group (E), Ap20 + HIR group (F). The Ap10 + HIR and Ap20 + HIR group showed a significant decrease in the lung IL-1β immune staining (blue arrows) in comparison to HIR group (red arrow) that showed a significant increase in the number of IL-1β immunoreactive lung tissue, there is no significant difference between the HIR group and Ap5 + HIR group (red arrow), and no significant difference between the Ap10 + HIR and Ap20 + HIR groups in comparison to the Sham and Sham + Ap10 groups (blue arrows). X200. The score of IL-1β immunohistochemical expression in lung in the studied groups (G) represent the mean ± SEM (n = 5–8). Results are considered significantly different when p < 0.05. asignificantly different from sham group. bsignificantly different from Sham + Ap10 group. csignificantly different from HIR group. dsignificantly different from Ap5 + HIR group. (Ap5: Aprepitant 5 mg/kg; Ap10: Aprepitant 10 mg/kg; Ap20: Aprepitant 20 mg/kg; HIR: Hepatic ischemia reperfusion; IL-1β: Interleukin—1β). Effect of different doses of aprepitant on lung NLRP3 inflammosome expression and TNF-α level in hepatic ischemia reperfusion-induced lung injury in rats were represented in Fig. 3H,I, respectively. Values represent the mean ± SEM (n = 5–8). Results are considered significantly different when p < 0.05. asignificantly different from sham group. bsignificantly different from Sham + Ap10 group. csignificantly different from HIR group. dsignificantly different from Ap5 + HIR group. esignificantly different from Ap10 + HIR group. (Ap5: Aprepitant 5 mg/kg; Ap10: Aprepitant 10 mg/kg; Ap20: Aprepitant 20 mg/kg; HIR: Hepatic ischemia reperfusion; NLRP-3: Nucleotide-binding oligomerization domain—like receptors protein 3; TNF-α: tumor necrosis factor alpha).
