Table 1 Primers used in the real-time qPCR.
From: IL-1β alters the virulence of uropathogenic Escherichia coli
Gene | Function | Forward primer (5’–3’) | Reverse primer (5’–3’) |
|---|---|---|---|
abf-2 | Microbicidal activity | CCGTTCCCTTTTCCTTGCAC | GACGACCGCTTCGTTTCTTG |
ape-1 | Apoptosis | GTTTGGTGATAGTCTAGACG | TGTTGTGGTATCACTACCTAATACC |
bar1 | Wnt signaling | CCTAATTTGCACGCTACGGC | TCGGCATGATCGGAATGGAG |
cep-1 | Apoptosis | AGGGCACGATTCAGTGTTG | TTCATCGCTTCCTGGATGC |
clec-60 | Antimicrobial defence | TGTCTGCATTCTTCCAGTCG | CCCATACCCAGACACCTTTG |
lys-7 | Antimicrobial response | ATTCAGGTCACTTCGCCAAC | ATCCGGTCGTGATCTGATTC |
tir-1: | Innate immune response | TTGGGTGCACAAAGAGCTGA | GGTCGGTGTCGTTCTGTTCA |
wah-1 | Apoptosis | GCTGATGCTGTCGAGGAGA | TGGTGGTGTTCTCTTCTGTAGA |
Y45F10D.4 (FES) | Fes, an iron-sulfur cluster assembly enzyme (endogenous control) | CTGGCAACCGAATGGATTA | CTTCTTAGTCTGCTTCTTCTGATA |
fimH | Type-1 fimbriae | GTGCCAATTCCTCTTACCGTT | TGGAATAATCGTACCGTTGCG |
papC | P fimbriae | GTGGCAGTATGAGTAATGACCGTTA | TATCCTTTCTGCAGGGATGCAATA |
grxA | Glutaredoxin1 redox coenzyme for glutathione-dependent ribonucleotide reductase | ATGAGCGCGATGATTTCCAG | CTCTTTCACCCATGCAGCAA |
purL | Phosphoribosylformyl-glycineamide synthetase | ACCTCGACTTTGCTTCCGTA | GCGCAGTTCAAATTTACCGC |
rlpB | A minor lipoprotein | GCCAAAGTCTTCCGTTCGTT | GTTGTGGTCGACGTCTGTTC |
nirB | Nitrite reductase | GAAGAACTGCTGGCGAAACA | CTTTCTGGATTGTGCGGAGG |
gapA | Glyceraldehyde-3-phosphate dehydrogenase (endogenous control) | AAGTTGGTGTTGACGTTG | AGCGCCTTTAACGAACATCG |
