Table 4 Antigenicity, allergenicity, toxicity, topology, solubility, sequence homology prediction and physico-chemical characteristics of the designed vaccine.

From: In silico design of novel multi-epitope peptide vaccine against Neospora caninum induced cattle abortion targeting extracellular GRA2 and Nc-p43 protein

Features

Results

Antigenicity

1.2156

Allergenicity

Non-allergen

Transmembrane topology

Outside

Solubility

0.948 (soluble)

Sequence Homology

< 25% query coverage

Number of amino acids

215

Theoretical isoelectric point (pI)

4.71

Molecular Weight

22529.66

Formula

C948H1503N283O334S10

Total Number of atoms

3078

(Asp + Glu)

38

(Arg + Lys)

26

Half-life

30 h (mammalian reticulocytes, in vitro)

> 20 h (yeast, in vivo)

> 10 h (Escherichia coli, in vivo)

Aliphatic Index

54.98

Instability Index

39.67

GRAVY

− 0.782

Solubility

0.948