Fig. 5

Sociability and motor stereotypies discriminate between backgrounds. Based on the behavioral phenotypes of B6 mice (green) and B6;129 mice (orange) in the reciprocal social interaction, three-chambered, motor stereotypies, Y-maze, open field, and novelty-suppressed feeding tests (A), principal component analysis revealed higher variability in B6;129 background in the first principal component (left), confirmed by a significant increase of the scores for the first principal component in B6;129 compared to B6 background (center). The most contributing behavioral parameters to this variability in B6;129 background were sociability and stereotyped behavior parameters (right). Based on the behavioral phenotypes of B6 (green) and B6;129 mice (orange) in the Live Mouse Tracker interacting with cage mates or unfamiliar age- and sex-matched B6 and B6;129 mice (B), the principal component analysis revealed increased variability of the B6;129 mice in the second principal component (left), evidenced by an increase in the scores for the second component (center). The most discriminating parameters contributing to this variability in the B6;129 background were social interaction parameters (right). Data are presented as individual data, mean ± sd (Tables S1-2). Groups were compared by the one-way ANOVA followed by Tukey’s posthoc tests, with stars indicating the background effect. *p < 0.05; **p < 0.01; ***p < 0.001.