Table 2 Regression parameters for determination of VON and ASP by the two proposed methods.
Parameters | HPTLC | HPTLC/IMAGE J | ||
|---|---|---|---|---|
VON | ASP | VON | ASP | |
Linearity µg/band | 1.0–10 | 5.0–25 | 1.0–10 | 5.0–25 |
Slope | 1840.09 ± 27.37 | 329.386 ± 5.31 | 923.007 ± 13.075 | 338.66 ± 6.30 |
Intercept | 2320.40 ± 170.96 | 1102.07 ± 88.06 | −328.758 ± 83.2881 | −580.96 ± 132.15 |
R | 0.9999 | 0.9999 | 0.9998 | 0.9999 |
LOD µg/band | 0.306 | 0.887 | 0.297 | 1.288 |
LOQ µg/band | 0.92 | 2.67 | 0.92 | 3.90 |