Table 4 Comparison of the developed HPTLC densitometric and smartphone-assisted HPTLC methods with previously reported methods for the determination of VON and ASP.
Method | principle | Linearity µg/mL | LOD µg/mL | LOQ µg/mL | Ecoscale score | Refs. | |||
|---|---|---|---|---|---|---|---|---|---|
VON | ASP | VON | ASP | VON | ASP | ||||
Spectrophotometric method | RD | 1–10 | 2–25 | 0.173 | 0.24 | 0.525 | 0.728 | 86 | |
1DD | 1–10 | 2–25 | 0.174 | 0.24 | 0.529 | 0.729 | |||
Chromatographic | HPLC | 0.5–10 | 1–100 | 0.17 | 0.33 | 0.5 | 1 | 82 | |
HPTLC | 0.1–2 µg/band | 0.1–10 µg/band | 0.03 µg/band | 0.03 µg/band | 0.1 µg/band | 0.1 µg/band | 77 | ||
Chromatographic | HPLC | 1–100 | 1–100 | 0.24 | 0.29 | 0.80 | 0.97 | 70 | |
Developed method | HPTLC | 1–10 µg/band | 5–25 µg/band | 0.03 µg/band | 0.03 µg/band | 0.1 µg/band | 0.1 µg/band | 80 | Our work |
