Table 1 Detection and quantification of Toxoplasma gondii genomic DNA in tissues collected from Eurasian beavers (Castor fiber) at post-mortem.

From: Occurrence and characterisation of Toxoplasma gondii infection in free-living Eurasian beavers, Castor fiber, from southern England

Beaver ID

Habitat

qPCR ratio (SQ T. gondii genome copies/SQ beaver genome copies)

Carcass autolysis

Brain

Heart

Liver

XT585-21

Free-living

NA

0.00005

Mild

XT607-21

Free-living

0.00013

0.00003

Severe

XT619-21

Free-living

0.00883

0.00225

0.00003

Mild

XT034-22

Free-living

NA

0.00002

Moderate

XT145-22

Free-living

0.00003

Mild

XT216-22

Free-living

NA

0.03928

Severe

XT415-22

Free-living

0.00158

0.00013

0.00006

Moderate

XT583-22

Free-living

0.00003

0.00005

0.00005

Moderate

XT584-22

Free-living

0.00051

0.00027

Moderate

XT040-23

Captive

0.00011

0.00008

Mild

XT053-23

Free-living

0.02768

0.00862

Moderate

XT145-23

Free-living

0.00001

Moderate

XT157-23

Free-living

0.00517

0.00028

Mild

XT189-23

Free-living

0.02444

Severe

XT451-23

Captive

0.00037

0.00050

0.00002

Mild

XT637-23

Free-living

0.00232

Severe

XT705-23

Free-living

0.11440

Severe

XT819-23

Free-living

0.00009

0.00015

0.00021

Severe

XT820-23

Captive

0.00168

Mild

  1. Each sample was first tested by nested PCR targeting a 529 bp repeat element within the T. gondii genome; positive samples were subsequently quantified by qPCR to assess suitability for genotyping, calculated as the number of T. gondii genome copies/ beaver genome copy. Samples represented by ratios > 0.001 were used for further genetic characterisation, highlighted in bold. Samples negative by nested PCR are indicated by “–” and samples that were not available to test are indicated by “NA”.