Fig. 4

Ancestral Area Estimation of Apteronotidae using a two time-step paleogeographic model of bioregion connectivity (LEM1b). Time-calibrated phylogeny from Tagliacollo et al. (2024). Pie charts represent marginal likelihoods of ancestral areas using the DEC model of geographic range evolution in BioGeoBEARS. Time slices: T1: 30—10 Ma; T2: 10 – 0 Ma. Bioregions in inset; B = Brazilian Shield, G = Guiana Shield, L = La Plata, N = Negro, O = Orinoco, T = Trans-Andean, W = Western Amazon. Macroevolutionary dispersal rate (δ) 0.028 in Apteronotini, and 0.082 in Navajini (Table 2).