Table 3 Single factor experiment on TFA production from enzymolysis of wheat bran.
Levels | Enzymolysis time/min | pH value | Solid-liquid ratio | Enzymolysis temperature/℃ | metal ion | Concentration of metal ion /mmol/L |
|---|---|---|---|---|---|---|
1 | 5 | 3.5 | 1:40 | 30 | Mg2+ | 0.5 |
2 | 10 | 4.5 | 1:35 | 40 | Ca2+ | 1.0 |
3 | 15 | 5.5 | 1:30 | 50 | K+ | 5.0 |
4 | 20 | 6.5 | 1:25 | 60 | Na+ | 10.0 |
5 | 25 | 7.5 | 1:20 | 70 | Fe2+ | 50.0 |
6 | 30 | – | – | – | – | – |
7 | 40 | – | – | – | – | – |
8 | 50 | – | – | – | – | – |
9 | 60 | – | – | – | – | – |
10 | 120 | – | – | – | – | – |
11 | 180 | – | – | – | – | – |
12 | 240 | – | – | – | – | – |
