Fig. 2 | Scientific Reports

Fig. 2

From: Rapid screening of ethylene glycol and diethylene glycol in raw materials and medicinal syrups using low-cost field deployable assays

Fig. 2

Determining EG using enzymatic assays. (a) Using alcohol dehydrogenase and aldehyde dehydrogenase, EG was found to convert at a far higher rate than the other alcohols. NADH was measured at 340 nm over 15 min. The dotted line shows the blank absorbance limit. (b) By additionally using glycolate oxidase to detect glycolic acid, EG could be determined more specifically. End-point absorbances were measured at 571 nm. (c) The detection of glycolic acid in alcohol samples, measured as the relative fluorescence unit (RFU). (d) Successful identification of EG simply by visual observation of the oxidised fluorogenic substrate as pink colour without the need for a plate reader. No pink colour was observed for the other alcohols. Error bars show the standard deviations of two replicates. Ordinary one-way ANOVA with Dunnett’s multiple comparison. **p < 0.01; ****p < 0.0001.

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