Fig. 1
FAM172A is upregulated in EOC and promotes cell migration, invasion, and proliferation. (A) Analysis of FAM172A expression in EOC versus normal ovarian tissues using the UCSC Xena database. (B) FAM172A protein levels in EOC tissues compared to adjacent normal tissues. (C) Immunohistochemical staining of FAM172A in EOC and matched normal ovarian tissues. (D) Kaplan–Meier survival curves for high (blue) and low (red) FAM172A expression groups; dashed lines indicate 95% confidence intervals. (E) Poor prognosis associated with high FAM172A expression. (F) FAM172A protein expression in EOC cell lines and a normal ovarian epithelial cell line. (G) Colony formation assay in A2780 and SKOV3 cells after FAM172A knockdown or overexpression; the bar graph shows the number of qualified colonies. (H) Western blot analysis of proliferation-related proteins (KI67, PCNA) following FAM172A modulation. (I) CCK-8 assay assessing the effect of FAM172A on EOC cell proliferation. (J) Wound healing assay evaluating migration ability after FAM172A overexpression or silencing. (K) Transwell assay examining migration and invasion capacities. (L) Western blot analysis of epithelial–mesenchymal transition (EMT)-related proteins after FAM172A overexpression or knockdown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
