Fig. 2
LRRK2 E193K variant promotes the enrichment of autophagosomes. (A) Electron micrographs of autophagic organelles from cryo-fixed human fibroblasts from wild-type and E193K carriers at steady state or upon exposure to NH4Cl (5mM, 24 h). For explanations of organelle lettering, see Supplementary Figure S2; autophagosome = arrow-heads, multivesicular late endosome = cross, lysosome = asterisk, immature autolysosome = arrow, degradative autolysosome = double arrows; white arrows mark corresponding organelles in the overviews (left and middle rows) and the respective enlarged frames (right row). Scale bars = 1μm. (B) Ultrastructural morphometry of relative frequencies (per 1000 square micrometer) of early maturation stages of autophagic organelles: autophagosomes/phagophores pooled and immature autolysosomes and (C) Ultrastructural morphometry of relative frequencies (per 1000 square micrometer) of mature, degradative autolysosomes, lysosomes and multivesicular late endosomes. Data are expressed as mean ± SEM, n = 3 (6–8 cells for each experiment). ***p < 0.001, #p < 0.05, ##p < 0.01,###p < 0.001. Two-way ANOVA.
