Fig. 2 | Scientific Reports

Fig. 2

From: In vivo, in silico effects of sakuranetin as a multi-target nutraceutical against PTZ-induced seizures via GABA restoration and BDNF/TrkB activation

Fig. 2

Effect of sakuranetin on A) latency to convulsion, B) seizure score, C) animal survival, and D) latency to fall off the rod in the rotarod test in PTZ-induced mice. Seizure severity was evaluated according to a modified Racine’s scale: stage 0, no response; stage 1, facial and ear twitching; stage 2, myoclonic jerks; stage 3, clonic convulsions of forelimbs; stage 4, rearing with forelimb clonus; and stage 5, generalized tonic–clonic seizures with loss of posture. Values are expressed as mean ± SEM (n = 6). Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc test for panels A–C (#P < 0.001 PTZ vs. control; *P < 0.05, **P < 0.01, ***P < 0.001 vs. PTZ) and one-way ANOVA followed by Tukey’s post hoc test for panel D (#P < 0.001 PTZ vs. control).

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