Table 2 Molecular markers and their PCR primers and programs used.
Loci | PCR primers | Sequence (5´- 3´) | PCR cycles | Ref. | ||
|---|---|---|---|---|---|---|
Denaturation | Annealing | Polymerization | ||||
TEF-α | F: EF4f R: Fung5r | GGA AGG GGA TGT ATT TAT TAG GTA AAA GTC CTG GTT | 94 °C: 2 min, 35 cycles 94 °C: 30 s | 53 °C: 30 s | 72 °C: 1 min 72 °C: 1 min 4 °C: ∞ | |
TEF1-α | F: EF4f R: EF3r | GGA AGG GGA TGT ATT TAT TAG TCCTCTAAATGACCAGTTTG | 94 °C: 2 min, 35 cycles 94 °C: 30 s | 53 °C: 30 s | 72 °C: 1 min 72 °C: 1 min 4 °C: ∞ | |
