Fig. 6 | Scientific Reports

Fig. 6

From: Mitochondrial proteomics reveals reductive metabolism dependent on glutamine in fibroblasts of idiopathic pulmonary fibrosis under hypoxia

Fig. 6

Validation of the glutaminase response under hypoxic conditions. (A) Western blot for GLS in total lysate (Control Normoxia n = 3 and IPF Normoxia n = 5. ® actin was used as a loading control. (B) Graph of the densitometric analysis. (C) Representative immunoblot of GLS and ® actin was used as a loading Control (normoxia and hypoxia) and IPF 1, 2 and 3 (normoxia and hypoxia). (D) Representative images showing the colocalization of α-SMA (red) and Ki67 (green) in control and IPF fibroblasts under normoxic and hypoxic conditions.

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