Fig. 5
Organization of Drosophila egg chambers and signal quantification. (A) Schematic representation of a Drosophila stage-10A egg chamber, oriented from anterior (left) to posterior (right). The oocyte, shown in orange, is connected to the anteriorly localized nurse cells, depicted in blue. It is surrounded by follicular cells in green. Within the oocyte, osk mRNA predominantly accumulates at the posterior pole, forming a crescent (dark orange). (B) Single confocal section of a stage-10A egg chamber probed with the acceptor AP-Cy5. The areas measured to calculate the SNR are outlined in white. From left to right: the osk mRNA crescent (signal) and 3 square areas within the follicular cells (background noise). (C) Graph illustrating the distributions of signal-to-background intensity ratios after hybridization with Ap only. The mean grey values of the Cy5 signal (ex. 633 nm, em. 650–700 nm) are derived from the FRET AP-Cy5 probes (left, shown in blue) and DRET A0P (right, in red). These ratios were determined from 15 egg chambers across three independent experiments identified by specific shades of blue (left) or red (right). The white horizontal lines indicate average values. Scale bar shown in (B) represents 20 µm.
